NATIONAL OPEN UNIVERSITY OF NIGERIA

UNIVERSITY VILLAGE, PLOT 91, CASDASTRAL ZONE, NNAMDI AZIKIWE EXPRESS WAY, JABI, ABUJA.
FACULTY OF SCIENCES
JULY 2018 EXAMINATION

BIO 305: Molecular Biology

Time allowed: 2hrs

Credit Unit: 2 units

Instruction: Answer four questions only. Question 1 is compulsory.

1a.      Itemize the components of Nucleotides and Nucleosides       (5 marks)

1b i.   Enumerate in a chronological order, the stages of transcription. (5marks)

1b ii.  List the Nonsense codons (3marks)

1c.i     List four occurrences in one turn of the Kreb’s cycle.(4marks)

1c.ii.  What are the two major functions of metabolic pathways? (3marks)

d.       State 5 techniques employed in Molecular Biology (5marks)

2a. Outline 7 characteristics of the Genetic code. (7marks)

2b. Mention the amino acids with the following codons.

i.UUU   ii. AAA   iii. CCC   iv. GGG   v. AGG   vi. GUG   vii. AUU   viii. GCC  (8marks)

3a. What are the requirements for the Sanger Method of DNA sequencing? (5marks)

3b. Describe the steps involved in Sanger Method of DNA sequencing. (10 marks)

4a. Define catabolism. (2marks)

4b. Outline the processes involved in glycolysis. (13 marks)

5. Justify the semiconservative nature of DNA replication. (15 marks)