NATIONAL OPEN UNIVERSITY OF NIGERIA

UNIVERSITY VILLAGE, PLOT 91, CASDASTRAL ZONE, NNAMDI AZIKIWE EXPRESS WAY, JABI, ABUJA.
FACULTY OF SCIENCES
NOVEMBER 2018 EXAMINATION

COURSE TITLE:     MOLECULAR BIOLOGY

CREDIT UNIT:         2

COURSE CODE:      BIO 305

TIME ALLOWED:   2 HOURS                  

INSTRUCTION: ANSWER FOUR QUESTIONS ONLY. QUESTION 1 IS COMPULSORY.

1a. Enumerate four characteristics of Escherichia coli that make it a suitable experimental model in Molecular Biology (6marks)

1b Highlight the roles of Deoxyribonucleic acid in organisms (6marks)

1c.Expantiate on the unique features of the transfer-Ribonucleic acid (8 marks)

1d. i Explain the term ‘Metabolism’ (3 marks)

ii. List the substances formed by pyruvic acid peculiar to;

1. The aerobic conditions (1 mark)
2. The anaerobic conditions  (1 mark)

 2a. State the role of Restriction Fragment Length Polymorphism, (RFLP) analysis in Molecular Biology (2marks).

2b. Describe the use of Gel electrophoresis in Molecular Biology. (13marks)

3a. Describe the structure of the chromosome (9 marks)

3b. Explain the role of chromosomes in hereditary (6 marks)

4. Describe the mechanism of chain initiation during protein synthesis (15 marks)

5a Describe the central dogma of Molecular Biology (6marks)

5b. Write at least 3 codons for  the following amino acids;

1. Proline   ii. Leucine   iii. Glycine (9 marks)