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NATIONAL OPEN UNIVERSITY OF NIGERIA
UNIVERSITY VILLAGE, PLOT 91, CASDASTRAL ZONE, NNAMDI AZIKIWE EXPRESS WAY, JABI, ABUJA.
FACULTY OF AGRICULTURAL SCIENCE
JUNE/JULY 2013 EXAMINATION
COURSE CODE: CSP 401
COURSE TITLE: Biotechnology in Crop and Pest Management
DURATION: 2 Hours
INSTRUCTIONS: Answer question 1 and any other 4 questions
Q1. I need to rapidly multiply an exotic banana cultivar for planting and ensure that the
resulting plant is identical to the stock plant material.
Discuss the general steps/stages that I should follow. 20 marks
Q2 (a) What is biotechnology? 5 marks
(b) Define the following terms:
(i) A Construct 5 marks
(ii) Cloning 5 marks
(iii) Restriction enzymes 5 marks
(iv) Transformation 5 marks
(v) Totipotency 5 marks
Q3. (a) Discuss the causes of mutation in two categories. 17 marks
(b) What is recombinant DNA? 3 marks
Q4. (a) What are transgenic plants? 3 marks
(b) Why are transgenic crops needed? 8 marks
(c) Discuss the three major genetic traits that are used in breeding for resistance to fungal diseases in crops? 9 marks
Q5. Discuss the GM control of insect pests. 20 marks
Q6. (a) List six ways through which herbicide resistance can be prevented or delayed.
0.5 x 6 points = 3 marks
(b) Discuss five advantages of herbicide resistant cultivars. 12 marks
(c) What is nitrogen fixation and why is the process essential for life? 3 marks
(d) What is denitrification? 2 marks
Q7 (a) Discuss the following genetic marker technologies:
(i) Restriction fragment length polymorphism 5 marks
(ii) Amplified fragment length polymorphism 5 marks
(iii) Random Amplification of Polymorphic DNA 3 marks
(b) What are the applications and limitations of the above technologies? 7 marks
