NATIONAL OPEN UNIVERSITY OF NIGERIA

UNIVERSITY VILLAGE, PLOT 91, CASDASTRAL ZONE, NNAMDI AZIKIWE EXPRESS WAY, JABI, ABUJA.
FACULTY OF AGRICULTURAL SCIENCE
OCTOBER 2013 EXAMINATION

COURSE CODE:                                                                              CSP 401                                                                             

COURSE TITLE:                                                                             Biotechnology in Crop and Pest Management

DURATION:                                                                                     2½ Hours

INSTRUCTIONS:                                                                            Answer question 1 and any other  4 questions

 

Q1.       Discuss biotic and abiotic constraints to crop production that have encouraged the application of biotechnology.

 

Q2.       Define the following terms:

(i)        Biotechnology           (ii)       Recombinant DNA    (iii)      Transgenic event

(iv)      A Clone                       (v)       Genetic engineering (vi)      Explant

            (vii)     A construct                (viii)    Biolistics                     (ix)      Totipotency

(x)       Restriction Fragment Length Polymorphism.

 

Q3       (a)       Discuss concerns over the development of herbicide tolerant crop

(b)       List six ways through which herbicide resistance can be prevented or delayed.

(c)       What is nitrogen fixation and why is the process essential for life?

 

Q4       (a)       What is a genetic marker?

(b)       List five commonly used genetic markers.

(c)       Discuss the three major genetic traits that are used in breeding for resistance to fungal diseases in crops.

 

Q5.       Discuss GM control of insect pest

 

Q6       (a)       What are the applications and limitations of Random Amplification of Polymorphic DNA

                        (RAPD) marker technology?

(b)       Discuss the three major genetic traits that are used in breeding for resistance to fungal diseases in crops.

Q7       (a)       Give six precautions that should be taken in order to get good response to

biofertilizer application.

            (b)       List four microorganisms that fix nitrogen.

            (c)       Distinguish between mutation and micropropagation.


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