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NATIONAL OPEN UNIVERSITY OF NIGERIA
UNIVERSITY VILLAGE, PLOT 91, CASDASTRAL ZONE, NNAMDI AZIKIWE EXPRESS WAY, JABI, ABUJA.
FACULTY OF AGRICULTURAL SCIENCE
OCTOBER 2013 EXAMINATION
COURSE CODE: CSP 401
COURSE TITLE: Biotechnology in Crop and Pest Management
DURATION: 2½ Hours
INSTRUCTIONS: Answer question 1 and any other 4 questions
Q1. Discuss biotic and abiotic constraints to crop production that have encouraged the application of biotechnology.
Q2. Define the following terms:
(i) Biotechnology (ii) Recombinant DNA (iii) Transgenic event
(iv) A Clone (v) Genetic engineering (vi) Explant
(vii) A construct (viii) Biolistics (ix) Totipotency
(x) Restriction Fragment Length Polymorphism.
Q3 (a) Discuss concerns over the development of herbicide tolerant crop
(b) List six ways through which herbicide resistance can be prevented or delayed.
(c) What is nitrogen fixation and why is the process essential for life?
Q4 (a) What is a genetic marker?
(b) List five commonly used genetic markers.
(c) Discuss the three major genetic traits that are used in breeding for resistance to fungal diseases in crops.
Q5. Discuss GM control of insect pest
Q6 (a) What are the applications and limitations of Random Amplification of Polymorphic DNA
(RAPD) marker technology?
(b) Discuss the three major genetic traits that are used in breeding for resistance to fungal diseases in crops.
Q7 (a) Give six precautions that should be taken in order to get good response to
biofertilizer application.
(b) List four microorganisms that fix nitrogen.
(c) Distinguish between mutation and micropropagation.
